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Cell culture and transfection HEK 293T (ATCC, #CRL-11268) and AGS (ATCC, #CRL-1739) cells were cultured in Dulbeccos modified Eagles medium (DMEM) supplemented with 10% fetal bovine serum (FBS, Gibco, USA) in the presence of 1% penicillin and streptomycin and incubated at 37 C in a 5% CO 2 atmosphere while MFC cells cultured in RPMI 1640 medium correspondingly