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Total RNA extraction and transcriptome sequencing analysis RNA-seq analysis was conducted by Novogene Co., Ltd (Beijing, China)

[25] In human cells with mutant WRN, NAD+ repletion restored normal mitophagy, improved fat metabolism, decreased mitochondrial oxidative stress, and enhanced mitochondrial quality

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This signal specifically suppresses the transcription of CYP7A1, the rate-limiting enzyme in classical bile acid synthesis, thereby preventing bile acid overload and hepatotoxicity (Kuang et al., 2023

A disruption in the homeostasis of iron metabolism, manifesting as an imbalance among its import, storage, and export, can lead to intracellular iron overload