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A magnified SEM image of 432 helicoid III (i), the corresponding three-dimensional model (ii) and the interpolated curved surface of 432 helicoid III (iii) are shown

indicating that this model has the best goodfit for predicting parasite density using serum GR activity in children ( Table 5 and Figure 2 )

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PIS was solubilized in buffer containing 0.9%(v/v) Triton X-100, 5%(v/v) glycerol, 0.5 mM DTT, 10 M PMSF, 30 mM MgCl 2 , 1 mM MnSO 4 , and 20 mM Tris-HCl pH 8.5