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(2024) Protocol for the in vitro reconstruction of site-specifically phosphorylated RNA Pol II to identify the recruitment of novel transcription regulators

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Injection process: Reconstitute the lyophilized powder with bacteriostatic water (see Reconstitution section below) Draw the appropriate dose into an insulin syringe (29 to 31 gauge) Pinch a fold of skin at the injection site Insert the needle at a 45 to 90 degree angle Slowly depress the plunger Hold for 5 seconds before withdrawing

The cells were washed and resuspended in ice-cooled phosphate-buffered saline in centrifuge tubes

This is a stressor for the body, causing hypoxia of intestinal cells due to redistribution of blood in the body, hyperthermia and dehydration [158, 159]