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doi: 10.1038/s41413-018-0016-9 64 McInnesIBSchettG

The corresponding residues in human IgG1 Fc, Ala330, and the soluble fragment of FcRIIIa, Ile106, were mutated, expressed individually and together in ExpiCHO cells, and purified using affinity and size exclusion chromatography (SEC)