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Based on these findings, Al Sabieh et al

After centrifugation at 18,000 rpm for 30 min, the supernatant was collected and incubated with glutathione-Sepharose 4B at 4 C, followed by washing with PBS buffer

This synthetic variant exhibits reduced binding to IGF binding proteins, allowing enhanced bioavailability and systemic activity [2]

IDT) per 1 ml media and incubated for 24 h before replacing media with DMEM with 10% FBS and 100 units/ml penicillin/streptomycin (15140122

We subsequently performed quantitative measurement of ONSD using standardized amplitude modulation (A-scan) echography with tissue sensitivity settings, placing the 8 MHz A-scan probe on the temporal eye equator in primary gaze position, as previously described by others [24, 25]