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The cell growth medium was replaced by a medium containing 2% FBS DMEM without sodium bicarbonate 1 h before the Seahorse MitoStress assay was performed

coli BL21 (DE3) cell lysate (0.5 mg/mL in 50 mM Tris buffer pH 7.5) incubated with [13C]-HCHO (6 mM) over time

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