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Using sterile water beyond a single use or sharing it between patients can risk contamination, since it has no agent to suppress bacterial growth

actual schedules in individual trial arms varied, with some cohorts taking up to 2436 weeks to reach the highest dose levels

The cell lines were cultured in DMEM medium containing 4.5 g/ml glucose and L-glutamine (Gibco) supplemented with 10% heat-inactivated fetal calf serum (Gibco) and 100 U/ml penicillin and 100 g/ml streptomycin (Gibco)

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