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The intake of antibiotics to somehow destroy the intestinal microbiota and the administration of the oligosaccharide lactulose, an osmotic laxative, can be additional methods

High abdominal pressure at 25, 30, 40, or 50 mmHg was maintained until sacrifice at 60 min (25 mmHg), 30 min (30 mmHg, 40 mmHg), or 15 min (50 mmHg)

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10.2147/jaa.S298613 7 BacanlM.AydnS.TanerG.GktaH

Investigator-assessed improvement Finding: The blinded investigator assessed the active treatment group as showing Scale 1 improvement (2650% improvement) at the final visit, while the placebo group showed Scale 0 improvement (025%)