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The homogenates were centrifuged at 20,400 g for 20 min at 4 C, and the resulting supernatants were appropriately diluted and used in the assays, which were conducted according to the manufacturers instructions

[DOI] [PMC free article] [PubMed] [Google Scholar] Ali RA, Qureshi MA, McCorkle FM (1994) Profile of chicken macrophage functions after exposure to catecholamines in vitro

ADH2 -overexpression in yeast To generate ADH2 -overexpressing strains (EG103 pGPD-ADH2 and EG110 pGPD-ADH2 ) the constitutive glyceraldehyde-3-phosphate dehydrogenase promoter (pGPD) was introduced to the endogenous ADH2 locus according to the method described by Janke et al