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After labeling, cells were quenched with 10 mM sodium ascorbate and 5 mM Trolox in DPBS, washed three additional times with DPBS and analyzed by Western blot or immunofluorescence as described below

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Some practitioners express caution about the appetite-suppressing mechanism of GLP-1 drugs in postpartum mothers, since adequate caloric intake supports both milk production and maternal recovery

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