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We hypothesized that our hydrogel formulations would maintain therapeutically relevant concentrations of TZP/Sema, comparable to daily 20 g TZP/Sema administration, for six weeks in this rat model

When in doubt, maintain your protocol and give the situation two to three more weeks before concluding something is wrong

doi: 10.1210/js.2019-00160

No, Freezing a reconstituted peptide causes ice crystals to form within the solution, and those crystals physically shear the peptide chain apart in a way that cant be reversed