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After centrifugation at 1000 x g for 10 min, the supernatant (S1) was centrifuged for 20 min at 17.500 x g to yield the supernatant S2 and the pellet P2 (crude synaptosomal fraction)

Glutamate transporters alterations in the reorganizing dentate gyrus are associated with progressive seizure activity in chronic epileptic rats