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10.1038/clpt.1987.207 4 AndersonK

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First, we assayed the ability of both proteins to undergo complex formation in HeLa cells co-expressing TBC1D9B-HA together with wildtype, active GTP-locked (Q75L), or inactive GDP-locked (T34N) variants of ARL8B-eGFP

The expression of ZO-1, Occludin, SIRT1, STAT3, p-STAT3, RORt, IL-6, IL-1, TNF-, and -actin was examined using the method previously described (Yao et al., 2014)