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However, injecting at the most fatty part of each area can improve absorption rate

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After washing with TBST, cells were stained in a mixture of 1 g ml 1 Alexa Fluor 594 goat anti-rat IgG (HL), 1 g ml 1 Alexa Fluor 488 goat anti-rabbit IgG (HL) and Hoechst 33342 in Can Get Signal Solution 2 for 30 min at 25 C