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After treatment, cells were immediately analyzed for apoptosis and the cell cycle using a BD FACSCelesta Cell Analyzer with an Annexin V-FITC/PI Apoptosis Detection Kit and PI/RNase Staining Buffer (BD Biosciences, Franklin Lakes, NJ, USA), respectively, according to the manufacturers instructions

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Prolotherapy has been shown to reduce pain and stiffness, and improve knee function and quality of life in knee OA patients

Finally, dicyclohexylcarbodiimide (DCC) effects the dehydration of a carboxylic acid and amine mixture to the corresponding amide under relatively mild conditions

Under resting physiological conditions, circulating levels of cTnT and cTnI are minimal