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Then, 1.5 mL of extraction buffer (80% methanol with 0.1% formic acid, v/v) was added, with vortexing for sample resuspension
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These include alterations in MEC II pyramidal cell projections to the hippocampal CA1 and cortical areas, as well as stellate cells targeting the dentate gyrus and CA3 regions, both of which are involved in memory and spatial navigation (Krishnan et al., 2025)
The DIAs in the Astral analyser were performed in centroid mode for a mass range of 380980 m/z with a window width of 2 Da (without overlap), a maximum injection time of 3 ms and a normalized AGC target of 500% after fragmentation using higher-energy collisional dissociation (25% normalized collision energy)
