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When a hexafluoroleucine was introduced at position 29 or 32, a moderate loss in binding affinity and biological activity was observed

Cells were then stained with DAPI for 30 min, washed with PBS (pH 7.4), fixed with 4% paraformaldehyde for 30 min, and mounted

Pre-existing pigmented lesions , including melanotic macules and oral naevi, may be the precursor in a proportion of cases, although most oral melanomas appear to arise without an identifiable precursor

( A ) Western blot analysis of sAC in bound and unbound fractions from pull-down experiments using streptavidin magnetic beads with biotinylated GLP-1(2836) and scrambled control incubated with mouse whole-heart lysates indicated no sAC binding to either peptide

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