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doi: 10.1038/s41586-021-03614-z

Briefly, cells were grown in YPD until logarithmic phase, harvested by centrifugation at 1,000 g for 3 min at room temperature and resuspended in 200 l One-step-transformation buffer containing 40% polyethylene glycol 3,350 (Sigma Aldrich, St

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This permits one to focus on lung airway epithelial cells and their response to the inflammatory process ( enu1 mouse and in the normal mouse treated IL-13 and GGsTop, although to a lesser degree