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The reduced form of glutathione (GSH) is a critical antioxidant for maintenance of the mitochondrial redox environment 28

To isolate chondrocytes, fresh articular cartilage tissues were rinsed with basal medium (BM), consisted of high glucose Dulbeccos modified Eagles medium (DMEM, Gibco/Thermo Fisher Scientific, Waltham, MA, United States), with 2 Antibiotics-Antimycotics (anti-anti, Life Technologies, Carlsbad, CA, United States) and then cut into 1 mm 3 pieces

doi: 10.3389/fpsyt.2018.00044 75

This age-related NAD+ depletion impairs mitochondrial energy production, reduces SIRT1 activity, compromises DNA repair mechanisms, and accelerates cellular senescence

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