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The tissue was fixed in 4%-paraformaldehyde (PFA) for 24 h, washed twice with PBS and dehydrated with ascending ethanol solutions, incubated with xylene and then embedded in paraffin

The observed metabolic-research differences between the two compounds are therefore not explainable by tirzepatide hits GLP-1 harder. They are explainable by the synergistic effect of dual-receptor engagement, where GIPR activation amplifies the downstream consequence of a lower-affinity GLP-1R hit

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