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Relative expression levels were determined by real-time PCR on a Bio-Rad CFX Connect or Real-Time System (CFX96 Real-Time System) using SYBR Green or the PowerUp SYBR Green kit (Thermo Fisher Scientific), and the primer sequences are listed in Supplemental Methods

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Dispute Over Timing of Discovery About Link Between Ozempic and Gastroparesis The drug makers have also urged Judge Marston to focus the first phase of discovery and motions practice on a third cross cutting issue, involving general causation, and whether plaintiffs can produce sufficient scientific evidence to establish that GLP-1 drugs are capable of causing the gastrointestinal injuries experienced by plaintiffs