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Histones were air dried then resuspended in 10 L of 0.1 M (NH) 4 HCO 3 for derivatization and digestion according to (Garcia et al., 2007)

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Due to differences in their pharmacokinetic profiles, in this exploratory experiment, we applied a higher dose of the GLP1/E conjugate (100 nmol/kg), relative to those of the dual (GLP1/GIP) or triple (GLP1/GIP/Glucagon) agonists (10 nmol/kg)