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Paired-end sequencing was performed on Illuminas NovaSeq 6000 sequencing system
For the quantification of sulfur metabolites in the tissue samples, the tissue metabolites were extracted and derivatized with NEM in ice-cold extraction solvent (80% MeOH: 20% H 2 O containing 25 mM NEM and 10 mM ammonium formate, pH=7.0) which includes stable isotope labeled internal standards (20 M [ 13 C 3 , 15 N]-cysteine-NEM, 36.4 M [ 13 C 2 , 15 N]-GSH-NEM, 10 M [D 4 ]-Cystine, 0.92 M [ 13 C 5 , 15 N 2 ]-GSSG, 20 M [D 4 ]-Hypotaurine, and 20 M of [ 13 C 2 ]-Taurine at a final concentration of 50 mg/mL followed by incubation on 4 C for 24 hr
Interactions of glucagon-like peptide-1 (Glp-1) with the blood-brain barrier
After an average follow-up of more than three years, participants receiving semaglutide showed a 20% reduction in major adverse cardiovascular events compared to those receiving placebo
doi: 10.1146/annurev.genom.1.1.507 157 MahleyR
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