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To directly validate the proposed roles of Vit A and Lup in sperm cryopreservation, future work could employ western blot analysis to quantify the expression and activation states of key proteins, such as NRF2 and Caspase 3, along with their downstream effectors, in sperm following freezethaw

These results provide a mechanism for the results reported by Chen et al

Table 1 Formation of major oxidants

Antioxidant and anti-inflammatory properties of curcumin, in The molecular targets and therapeutic uses of curcumin in health and disease,

However, in individuals with a confirmed or subclinical B12 deficiency, correcting that deficiency can lead to the following: Improved energy levels and reduced fatigue Better exercise tolerance and recovery More stable mood and motivation Improved sleep quality These are indirect but meaningful contributors to weight management