FIGURE 8 3.8 Immunohistochemical findings The immunohistochemical investigation demonstrated a significant increase in expression of proapoptotic makers, Bax, and caspase-3, in MTX treated group, which was associated with decreased antiapoptotic marker, Bcl-2, expression when compared to the CTRL group (Figure 9)
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A key controller of this cellular defense mechanism is the Nrf2 (nuclear factor erythroid 2related factor 2)-antioxidant response element (ARE) pathway
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The overall strategy is illustrated in Scheme 1