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Practical limits depend on sterile techniquemost research protocols target fewer than 30 punctures per vial

In accordance with recommendations of nutrient and energy supply of the Society of Nutrition Physiology (GfE), the cows were fed a partial mixed ration, consisting of 50% roughage (70% maize silage and 30% grass silage) provided in feed-weigh troughs (Roughage Intake Control System Insentec B.V., Marknesse, The Netherlands) and 50% concentrate feed, provided in electronic concentrate feeding stations (Insentec B.V., Marknesse, The Netherlands)

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