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Mobile phase composition, gradient parameters and column chemistry may significantly influence retention time and separation efficiency

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Front Physiol 9:1167 Ochoa-Callejero L, Garca-Sanmartn J, Villoslada-Blanco P, iguez M, Prez-Matute P, Pujadas E, Fowkes ME, Brody R, Oteo JA, Martnez A (2021) Circulating levels of calcitonin gene-related peptide are lower in COVID-19 patients

Receiver operating characteristic (ROC) analysis was subsequently used to explore the discriminative performance of selected metabolites between pre- and post-intervention states (Figs