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Specifically, in-house donors exhibited lower NAD + , NADH and NADP + levels (on average 3.2, 0.47 and 1.6 uM lower, respectively) but higher NADPH levels (on average 0.88 uM) compared to biobank samples (Supplementary Figure 2a)

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In vitro glucose-stimulated insulin secretion (GSIS) Insulin secretion was measured using the Krebs Ringer Bicarbonate (KRB) buffer (119 mM NaCl, 5 mM KCl, 2.5 mM CaCl 2 , 1.2 mM KH2PO 4 , 1.2 mM MgCl 2 , 10 mM HEPES, 25 mM NaHCO 3 , and 0.2% BSA)

Genetics can also play a role