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Supports cellular energy production and metabolism

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Due to the small number of smokers, the correlation between smoking and oxidative stress parameters was not evaluated, neither was the correlation between alcohol and oxidative stress parameters, due to the low consumption of alcohol reported

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eWAT explants (500 mg) were digested in 1 mL DMEM supplemented with 2% fatty acidfree BSA (Sigma-Aldrich, 126575), HEPES (10 mM), Liberase TM (thermolysin medium) (25 g/mL) (Roche, 05401119001), and DNAse (250 g/mL) (Roche, 10104159001) for 1 hour at 37C