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In vitro LOXL2 BHZ binding assay A7r5 cells were transduced with adenovirus in serum-free insulin-transferrin-selenium (ITS)-supplemented DMEM for 48 h to overexpress LOXL2 or LOXL2-DM

This baseline allows objective comparison at three, six, and twelve months

Buccellato FR, DAnca M, Fenoglio C, Scarpini E, Galimberti D (2021) Role of oxidative damage in Alzheimers disease and neurodegeneration: from pathogenic mechanisms to biomarker discovery

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All these e-GSTs display an extraordinary stability at 4 C except for the pig e-GST that began to lose activity after 2 days (Supplementary Table S2)