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(2) molecular dynamics simulated annealing restraining FOXO4 FH (without flexible regions) harmonically to its template structure (using a non-crystallographic force constant of 10,000 kcal mol 1 2 ), with additional dihedral angle restraints from 1 H, 15 N, and 13 C chemical shifts using TALOS-N 56 , hydrogen bond restraints (region p53 residues 48-55

Based on the effectiveness of ferroptosis and pyroptosis combined induction, in vivo experiments have confirmed that dual-induction nanoparticles demonstrated ideal anti-cancer effects in a xenograft mouse model, suggesting that the combined induction of ferroptosis and pyroptosis may be an effective and promising cancer treatment approach (179) ( Figure 8D )

(November 2010)

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