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After washing, cells were incubated with FITC-labeled goat anti-rabbit IgG (H + L) (Beyotime, Shanghai, China) and DAPI for nuclear staining

The advantage of this drug is that it does not target the isoform B of the IR which is involved in glucose metabolism and therefore does not induce the hyperglycaemia and metabolic toxicity observed with IGF-1R targeted therapies or TKIs (Hussain et al., 2019)

The increase of fluorescence due to GSH conjugate was converted to the corresponding concentration of GSH in the reaction mixture since 1.0 fluorescence unit recorded on the chart had been calibrated to correspond to 0.1 M concentration of GSH conjugate

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