In brief, HUVECs were lysed in the Co-IP buffer containing 20 mM Tris-HCl (pH7.5), 100 mM NaCl, 0.5 mM EDTA, 0.5% NP-40, 0.5 mM PMSF, 0.5% protease inhibitor cocktail on ice for 20 min
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In sum, the content presented in this review demonstrates that there have been significant leaps in our understanding of the GM, the factors influencing its composition and diversity, and its role in influencing host health through regulating metabolism and immunity
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