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doi:10.1096/fasebj.22.1_supplement.445.8

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The trapped ammonia was determined through titration with 0.1 N HCl and the burette reading was used to calculate the percentage of nitrogen using the formula - The percentage of nitrogen calculated was used for the calculation of total protein content using the conversion factor of 5.81

Hahne H, Pachl F, Ruprecht B, Maier SK, Klaeger S, Helm D, et al