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139 However, long-term administration of rapamycin has significant side effects (including skin and metabolic disorders) and inhibits mTORC2

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Colony replating assay After performing the clonogenic assay as indicated above, cells in the colonies ( n = 3 plates for each condition) were de-attached, and the viable cells were counted, showing similar and representative reductions on absolute viable cell counts (GUAVA easycyte) as previously observed in the number of colonies (Supplementary Fig

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