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In addition, there is also an increased risk of skin infections with this method

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Diagnosis requires careful clinical assessment, including detailed pain history, neurological examination, and sometimes specialized sensory testing to characterize the pain response patterns and identify underlying causes

Levels of HMGB1::Lucia in the supernatant can be readily monitored by measuring the light signal produced after the addition of QUANTI-Luc 4 Lucia/Gaussia, a Lucia luciferase detection reagent ( see figure )