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An ITC was conducted using the Bucher method to compare the relative cardiovascular efficacy of tirzepatide to semaglutide (subcutaneous or oral preparations) via the common comparator of placebo in people with T2D and established CVD

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Then the cells were centrifuged and washed once with 1 ml of 0.1 M borax (Sigma-Aldrich, 71997), followed by another wash with 1% BSA in PBS

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