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HZGHC001085c001) human haploid (HAP1) cells, created using CRISPR-Cas9 technology were purchased from Horizon Discovery Inc (Vienna, Austria)

Copper Chelation and Delivery At the most fundamental level, GHK functions as a copper chelator and delivery system - the tripeptide binds Cu with exceptionally high affinity (binding constant approximately 10 M, among the highest known for biological copper chelators), forming a square planar coordination complex where the copper ion is coordinated by the nitrogen atoms of the terminal amino group, the two peptide bonds, and the imidazole nitrogen of histidine

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Toll-like receptors (TLRs) play a key role in microorganism and viral particle recognition and activation of the innate immune system (Sasai and Yamamoto, 2013