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Briefly, the purified WD40 domain was provided as the analyte with a buffer composition of 20 mM Tris-HCl (pH 7.5) or 50 mM MES-NaOH (pH 6.5), 150 mM NaCl, 5 mM DTT, 10% glycerol, 0.2 mg/ml bovine serum albumin (BSA), and 0.002% Tween 20

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As an antioxidant, it prevents damage to important cellular components that arise due to reactive oxygen species like free radicals and peroxide

2021 Jul [cited 2026 Jan 28];14(7):22

The assays were conducted on the brain homogenate using rat ELISA kits, all sourced from ABclonal Technology, Woburn, MA, United States, in conformity with the manufacturers instructions