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The nuclei were washed with wash buffer (10 mM HEPES pH 6.5, 10 mM EDTA, 0.5 mM EGTA, and 0.25% Triton X-100) three times and resuspended in ice-cold nuclei lysis buffer (50 mM Tris-HCl pH 8.0, 10 mM EDTA, 1% SDS and 1 protease inhibitor cocktail), followed by sonication for six cycles (10 s ON and 30 s OFF) using the Bioruptor (Diagenode)

(3) Because vitamin B12 and folate work closely together, many healthcare professionals recommend evaluating both nutrients when symptoms of deficiency are present

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