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Samples were analyzed in triplicate and normalized to total protein that was determined using the Bradford Protein Assay Kit (Thermo Scientific, Rockford, IL)

Therefore, some of these findings would corroborate that UA may be a modifiable factor in the progression of chronic kidney disease, without sufficient data to state whether the beneficial effect of allopurinol is due to the reduction of UA or to the antioxidant effect produced by inhibiting the xanthine oxidase enzyme, however, the two large randomized clinical trials do not offer positive results so there is insufficient evidence to recommend allopurinol treatment in patients with asymptomatic hyperuricemia

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Post-market safety surveillance matters because rare problems may only become visible after many people use a product in real life