Briefly, approximately 1.510 5 host cells (A549, RAW 264.7, PBL-985, and PBL-985 nox2 -KO cell lines) were seeded per well in 12-well plates and cultured in DMEM + 5% FBS at 37C and 5% CO2 for 24 h until reaching 95% confluence, equivalent to approximately 3 10 5 cells
Intestinal permeability test Mice were orally administered 0.5 mL of 25 mg/mL 4 kDa fluorescein isothiocyanate (FITC)-dextran (Merck, 46,944) in sterile water 30 min prior to sacrifice
Consistently, the GSH-inducible expression of defense marker gene OPR3 , JAZ5 , and JAZ10 was significantly repressed in the GCaMP3/glr3.3 , but not obviously inhibited in the GCaMP3/glr3.6 and the GCaMP3 plants (Fig
Regulating leukocyte adhesion Furthermore, SDC-1 dynamically regulates leukocyte adhesion through chemokine anchoring, offering a dual role in inflammation control [50, 51]
doi: 10.3389/fphar.2022.891535 336 MahmoudGAERashedNMEl-GanainySMSalemSH