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Mitochondrial mass by NAO staining ( i , n = 3 biological replicates) and mitochondrial imaging by TOM20 staining (representative images from n = 2) ( j ) in U2OS cells treated 5FU for 72 h, with mean s.e.m

Intriguingly, we found that NAPstar4.3 had a strongly decreased affinity for NADPH, K d (NADPH) = 42.6 M, K r(NADPH/NADP+) = 0.28 ( Figure 1C,E ), and no detectable affinity for NADH ( Figure 1D,E)