The system biology of thiol redox system in Escherichia coli and yeast: Differential functions in oxidative stress, iron metabolism and DNA synthesis
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Treatment of Arabidopsis seedlings with 5 mM H 2 O 2 increased GCL activity and resulted in a shift from the less active reduced form to the more active oxidized form, as determined by activity assays and immunoblot analysis (Hicks et al., Conservation of the disulfide linkage that mediates the monomer/dimer transition of GCL occurs across the plant kingdom (Gromes et al., Nicotiana tabacum ), Agrobacterium tumefaciens (a-proteobacteria), and Xanthomonas campestris (-proteobacteria) showed that the two proteobacterial GCL remain monomeric during redox changes, even though the disulfide is retained
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The column temperature was increased from 100 C for 1 min, raised at 10 C /min to 200 C, then at 6 C /min to 280 C (3 min hold)
