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Cysteine and cysteine-dependent metabolites (glutamylcysteine, GSH and hypotaurine) were labelled when cells were grown in medium with 13 C-cystine and became unlabelled when cancer cells were grown in cystine-free medium with unlabelled GSH (Fig

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Then, the unfolded substrate is translocated to a protein hydrolysis region isolated from the surrounding aqueous environment