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The more relevant research question is whether triple receptor agonism (GIP + GLP-1 + glucagon) combining elements of both approaches could outperform either dual agonist

Western blot analysis RIPA Lysis Buffer (TransGen, Beijing, China) was used to extract total protein from tissues/fibroblasts and a Nuclear and Cytoplasmic Protein Extraction Kit (TransGen) was used to extract the protein from nucleus and cytoplasm of fibroblasts