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The RNA concentrations were measured using a ThermoFisher Scientific Nanodrop TM spectrophotometer, and 12 g of total RNA was then used to synthesize cDNA using High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems, 4368814) according to the manufacturers recommendations

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Basis size ( k x ) for the neonicotinoid smooth term ( k 1 ) was set to four, and for the DEM smooth term ( k 2 ), it was set to three, i.e., the maximum size for this experimental design

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