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PCR assays were performed using StepOnePlus (Applied Biosystems, California, USA)

Reverse dimerization assay HeLa cells were co-transfected with the Golgi marker GalT-mRFP (not displayed in all figures) and Go-Nt 7 -FM 4 -GFP, MGNC-Nt 7 -FM 4 -GFP or GFP-FM 4 -hGH (at a 1:3 plasmid ratio), and seeded on poly-L-lysine-coated coverslips for 15 h as indicated above

The principal component analysis (PCA) score plot for HILICpos, HILICneg, and C18pos showed that the distribution for study quality control samples, which mixed all aqueous humor samples, was observed in the center, indicating that the analysis had high quality (Supplementary Fig

However, GH is a potent growth signal and must be managed carefully