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Conflict of interest FG received research support from Eli Lilly, LifeScan, and Takeda and served as a consultant and author for AstraZeneca, Boehringer Ingelheim, Eli Lilly, Merck Sharp and Dohme, Novo Nordisk, Roche Diabetes Care, and Sanofi

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The authors would like to gratefully acknowledge the training and use of mass spectrometry instrumentation provided by Professor Perdita Barrans group at the Michael Barber centre for collaborative mass spectrometry (MBCCMS) based at the University of Manchester